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Assay
Procedure and Principle
Kit
Components
MTA™
The MTA™ drug test system contains materials for 192 results. The number of drug panels per kit will vary depending on the number of analytes per panel. For example, with 12 drugs per panel each test provides results for 16 panels; with 6 drugs per panel each kit provides results for 32 panels.
Included in the MTA™ kit are :
- 2 antibody coated microtiter plates. (Each plate contains 12 color coded microwell strips. Each color refers to a specific immobilized antibody for a drug analyte.)
- A single combination enzyme
conjugate reagent for all 12 drug conjugates.
- A rinse solution
concentrate.
- A single substrate/chromogen
reagent.
- A stop solution.
- A package insert.
- A lot specific profile
card.
Single Drug
EIA Kits
Venture Lab Inc.'s drug EIA kits contains testing materials for 192 tests (2 plates). Included in each kit are:
- 2 antibody coated microtiter
plates.
- An enzyme conjugate
reagent.
- A rinse solution
concentrate.
- A single substrate/chromogen
reagent.
- A stop solution.
- A package insert.
Assay
Procedure
All Venture Labs drug ELISAs including the MTA™ assays follow the same simple microtiter ELISA procedure:
1. Place the desired number of coated wells in the holder and secure them. (Note : a series of different colored wells is required for each specimen run in the array mode).
2. Dispense 30 uL of standard or sample into each series of
twelve different colored wells.
3. Add 75 uL of enzyme conjugate reagent to each well. Mix
and incubate 30 minutes at room temperature.
4. Empty the contents of the plate and with running cold tap
water fill and empty the plate contents (ten cycles).
5. Fill the wells with rinse buffer, empty and pound the
plate over an absorbent surface. (Dry the outer surface of
the plate.)
6. Add 100 uL of substrate/chromogen reagent to each well.
Mix and incubate 15 minutes at room temperature.
7. Add 50 uL of stop solution.
Assay Principle
Venture Labs Inc.'s drug test assays
are solid phase enzyme immunoassays that rely on competition
between free drug in the sample and drug bound on the enzyme
conjugate for antibody fixed on a microtiter plate
well.
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1.
Drug in the sample or calibrator and drug labeled
enzyme conjugate compete for binding sites on the
antibody immobilized on the surface of the
microwell during the first
incubation.
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2. The
plate is washed, removing any excess, unbound
enzyme conjugate. Substrate is added, and color is
developed.
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3. The
reaction is stopped, and the plate is read at 450nm
on a plate reader. The absorbance (color) is
inversely proportional to the amount of drug in the
sample.
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